TRAVISTRA

 THIN LAYER CHROMATOGRAPHY It is a chromatographic separation method used to separate mixtures, to check the purity of a mixture, and to monitor the progress of the reaction. Procedure A glass plate coated with a material of the adsorbent will constitute the stationary phase. The material is made into a slurry or a paste and the glass plate is coated with it by a thin layer applicator and dried. This glass plate with a thin layer of stationary phase is called chromatoplate. The mixture to be separated is applied to one end of the plate and it is placed in the solvent. When the solvent reaches near the top, it is removed and dried. If the components are colored the spots can be readily located. If the components are colorless the dried plate is sprayed with a suitable reagent to make the spot colored. In this way, the position of the components is located and their Rf values are determined. Rf value is the ratio of the distance traveled by a component to the distance traveled by the...

BEER LAMBERTS LAW   When a beam of monochromatic EM radiation is passed through an Absorbing solution of concentration (c), the rate of decrease in intensity(-dI) of radiation with a thickness of the solution (dx) is proportional to the intensity of the radiation (I) and concentration (c) of the solution. -dI/dx ∝ Ic  -dI/dx = kIc  -dI/I =kcdx Beer Lambert's law gives the linear relationship between the absorbance of EM radiation and the concentration of an absorber. A = εcL A is the absorbance c is the concentration L is the path length Ε is the molar extinction coefficient