TRAVISTRA

 GAS CHROMATOGRAPHY It is used for the separation as well as analysis of gaseous mixture and volatile organic compounds. Principle The components of a mixture are separated depending upon the extent of adsorption or partition of the stationary phase and at the rate at which the component is carried out by the mobile phase.  The mobile phase is a gas like Argon, Nitrogen, Helium, or hydrogen. The stationary phase may be solid or liquid. If the stationary phase consists of a solid material like silica, alumina, then chromatography is termed as Gas Solid Chromatography (GSC), and if the stationary phase is a liquid held as a thin layer on a solid support, then the technique is known as Gas-Liquid Chromatography (GLC)GSC- adsorption. GLC- partition INSTRUMENTATION  Carrier gas: It is allowed to flow through the system, carrying the sample in the vapor state through the column. It should be chemically inert, suitable for the detector employed, it should give the best column pe...

 HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY It is a method used for the separation, purification, and identification of various nonvolatile high molecular weight organic compounds and natural products like cholesterol, terpenoids. ADVANTAGES High-speed separation Excellent column separation Solvent consumption is minimum Sensitive and accurate Separation can be done at an ambient temperature. PRINCIPLE The components of the mixture are separated depending upon the extent of adsorption and partition on the stationary phase and the rate at which the component is carried by the mobile phase. The mobile phase is liquid and the stationary phase can be solid or liquid. INSTRUMENTATION Solvent reservoirs are used to collect the different solvents. Pressure pump to apply pressure  Sample injector to give the mixture which is to be separated in the analytical column Regulator to adjust the flow rate Guard column to remove the particulate matter and contaminants from the solvent. The analyt...